Refolding of subtilisim BPN' achieved almost quantitatively by covalent immobilization on an agrose gel.

Immobilization of Subtilisin Carlsberg on Modified Silica Gel by Cross-linking and Covalent Binding Methods

Proteases are important enzymes that their role in various industries is undeniable. However, keeping enzymes stable during its activity in harsh conditions is so important. In this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and pHs was evaluated. First silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

immobilization of subtilisin carlsberg on modified silica gel by cross-linking and covalent binding methods

proteases are important enzymes that their role in various industries is undeniable. however, keeping enzymes stable during its activity in harsh conditions is so important. in this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and phs was evaluated. first silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

Refolding proteins by gel filtration chromatography.

We have developed a facile means for the refolding of milligram quantities of purified proteins that employs gel filtration chromatography. We demonstrate by electrophoretic mobility shift and NMR spectroscopy that human ETS-1 protein, bovine ribonuclease A and E. coli integration host factor can be refolded into the native conformation using this technique. We have extended this strategy to th...

Covalent Immobilization of Sapindus Peroxidase on Polyaniline

Covalent immobilization of peroxidase is convenient in continuous processes to improve them economically. In the present study, peroxidases from Sapindus mukorossi were immobilized and used for removal of 4-chlorophenol (4-CP) by the polymerization pathway. Five milligram of vacuum dried polyaniline-glutaraldehyde composite was incubated in 1ml sodium acetate buffer (50mM;pH 5.0) containing 0.5...

Site-Directed Covalent Immobilization